Journal: Biochemistry and Biophysics Reports

Article Title: Activation of the AKT-mTOR pathway confers selpercatinib resistance in thyroid cancer cells harboring the CCDC6-RET fusion gene

doi: 10.1016/j.bbrep.2025.102136

Figure Lengend Snippet: Effect of the RET inhibitor selpercatinib on the growth of parental TPC-1, selpercatinib-resistant TPC-1 (TPC-1/SELR), and CUTC48 cells. Sensitivity to selpercatinib was determined using a WST assay. The graph shows cell viability of all three cell lines at different selpercatinib concentrations. Error bars represent the standard deviation (∗ p < 0.05, ∗∗ p < 0.01, vs. TPC-1). The table shows IC 50 (nM) and relative resistance ratio for selpercatinib obtained from three independent experiments.

Article Snippet: Gene sequence analyses of TPC-1, TPC-1/SELR, and CUTC48 cells were performed by DNA Chip Research Inc. (Tokyo, Japan).

Techniques: WST Assay, Standard Deviation

Journal: Biochemistry and Biophysics Reports

Article Title: Activation of the AKT-mTOR pathway confers selpercatinib resistance in thyroid cancer cells harboring the CCDC6-RET fusion gene

doi: 10.1016/j.bbrep.2025.102136

Figure Lengend Snippet: Expression and phosphorylation of signal transduction–related proteins at steady state in parental TPC-1, TPC-1/SELR, and CUTC48 cells. Representative images of western blots are shown (left panel). “p-” indicates phosphorylated protein. Overall, 10 μg of protein was loaded on each lane. β-actin was used as a loading control. The protein expression levels were normalized against β-actin, and phosphorylation levels against the level of expression of the corresponding protein. The relative densitometry values are stated below each protein band. Bar charts show the relative expression levels to TPC- 1 calculated from three independent experiments (right panel). Data are presented as mean ± SD (∗ p < 0.05, ∗∗ p < 0.01, vs. TPC-1).

Article Snippet: Gene sequence analyses of TPC-1, TPC-1/SELR, and CUTC48 cells were performed by DNA Chip Research Inc. (Tokyo, Japan).

Techniques: Expressing, Phospho-proteomics, Transduction, Western Blot, Control

Journal: Biochemistry and Biophysics Reports

Article Title: Activation of the AKT-mTOR pathway confers selpercatinib resistance in thyroid cancer cells harboring the CCDC6-RET fusion gene

doi: 10.1016/j.bbrep.2025.102136

Figure Lengend Snippet: Effect of RET inhibitor selpercatinib on protein expression in parental TPC-1, TPC-1/SELR, and CUTC48 cells. Expression and phosphorylation of RET, EGFR, MEK, ERK, AKT and mTOR in these cells 24 h after treatment with selpercatinib (0, 1, and 10 nM) were analyzed using Western blot. β-actin was used as a loading control, and proteins were loaded at 10 μg/lane. Representative images of western blots are shown (left panel). The protein expression levels were normalized against β-actin, and phosphorylation levels against the level of expression of the corresponding protein. The relative expression or phosphorylation levels are disclosed below each protein band. Relative expression or phosphorylation levels calculated from three independent experiments are presented as a bar chart (right panel) (∗ p < 0.05, ∗∗ p < 0.01, vs. 0 nM of selpercatinib).

Article Snippet: Gene sequence analyses of TPC-1, TPC-1/SELR, and CUTC48 cells were performed by DNA Chip Research Inc. (Tokyo, Japan).

Techniques: Expressing, Phospho-proteomics, Western Blot, Control

Journal: Biochemistry and Biophysics Reports

Article Title: Activation of the AKT-mTOR pathway confers selpercatinib resistance in thyroid cancer cells harboring the CCDC6-RET fusion gene

doi: 10.1016/j.bbrep.2025.102136

Figure Lengend Snippet: Effect of mTOR inhibitor everolimus on growth and protein expression in parental TPC-1, TPC-1/SELR, and CUTC48 cells. A. The sensitivity to everolimus was verified using WST assays. Data are represented as mean ± standard deviation. B. Expression and phosphorylation of RET, EGFR, MEK, ERK, AKT and mTOR in these cells 24 h after treatment with everolimus (0, 1, and 10 nM) were analyzed using Western blot. β-actin was used as a loading control, and proteins were loaded at 10 μg/lane. Representative images of western blots are shown (left panel). The protein expression levels were normalized against β-actin, and phosphorylation levels against the level of expression of the corresponding protein. The relative expression or phosphorylation levels are shown below each protein band. Relative expression or phosphorylation levels calculated from three independent experiments are presented as a bar chart (right panel) (∗ p < 0.05, vs. 0 nM of everolimus).

Article Snippet: Gene sequence analyses of TPC-1, TPC-1/SELR, and CUTC48 cells were performed by DNA Chip Research Inc. (Tokyo, Japan).

Techniques: Expressing, Standard Deviation, Phospho-proteomics, Western Blot, Control